DOLPHIN: Nano-Bio-Materials for Life group

Functionalized anthropogenic nanoparticles

The group is interested in the controlled synthesis and/or development of methods for the synthesis and functionalization of nanoparticles of controlled size, shape and properties that will be used alone or integrated into a medical device.
The aim is to develop anthropogenic (organic or inorganic) functionalized nanoparticles in a controlled and safe manner, for applications in nanomedicine - multifunctional "core-shell" systems and/or functionalized magnetic nanoparticles for theranostics; "natural soft" nanoparticles for the delivery of active ingredients: nanoliposomes (in collaboration with the LiBio/ENSAIA/UL, carrier of the theme) or polysaccharide-based nanocapsules for controlled drug delivery, targeting diseases and/or tissue regeneration.

Thesis:

• Pierre Venturini (2014- 2017)
• Thomas Girardet (financed by MESRI / in progress)
• Adrien Yepseu    (Codirection with Cameroun / in progress)
• Charles Roux-Pertus (financed by DGA)

Projects:

• Project Region - CNRS: Controlled development and characterization of functionalized iron oxide nanoparticles for biomedical applications
• PEPS Mirabelle +: SoilMagnetic Signature Oscillation: A proxy to stimulate Metal Bioavailability" (SIGMABIO) Carrier: ZegeyeAsfaw (LIEC) (2019-2022)

Articles :

• Carbon Nanostructures in Cancer Diagnosis and Therapy, J Nanom Nanos Tech: JNNT-110. (2019), Bendjemil B, Cleymand F, Pichler T, Knupfer M, Fink J.
   
• In situ Glyco-nanostructure formulation via photo-polymerization induced self-assembly, Polymer chemistry 9, 2868-2872, (2018), K Ferdji, P venturini, F Cleymand, C Chassenieux, JL Six.
   
• Facile One‐Step Synthesis of Polyoxazoline‐Coated Iron Oxide Nanoparticles, ChemistrySelect, Vol.3(42), p.11898-11901, (2018), S Fleutot, F. Cleymand, T Hauet, JC Dupin, P. Venturini, J. Ghanbaja, H. Martinez, JJ Robin, V. Lapinte.
   
• Nanoliposomes of marine lecithin, a new way to deliverTGF-β1, Journal of Biomaterials and tissue engineering 7,11, 1163-1170, (2017), G. Dostert, C. J. F. Kahn, P. Menu, B. Mesure, F. Cleymand, M. Linder, É. Velot, E. Arab-Tehrany.

Bioinspired biomaterials for tissue engineering and regenerative medicine

Nature is inspiring the development of new classes of biomaterials and processes, combining tools from chemistry, physics, materials science and engineering.

The group designs and produces biomaterials that are artificial and/or encompass natural products (development and optimization of extraction directly from the agro-resource) that closely mimic the physico-chemical, mechanical and biological properties of natural and/or living systems.

Research focuses on the design and manufacture of advanced biomimetic systems. These can be adapted to provide new and innovative solutions in the field of functional, bioactive, biodegradable medical devices. This research has applications in personalized medicine, such as tissue engineering.

The group is also exploring the use of layer-by-layer assembly and 3D/4D bio-printing (development of bio-inks) to develop scaffolds with controlled multi-scale architecture capable of exposing cells to personalized micro-environments. The use of nano/micro technologies (and their development) enables

Thesis:

A Poerio (coll WFI/USA and CICECO/Portugal), funding for a LUE PhD

Projects:

• 2019-2021: the 2019 Gutenberg Chair was obtained by Pr JF Mano entitled “CARDIOGEL – Advanced bioinspired hybrid hydrogels for cardiac regeneration”
   
• 2018-2021: Professor@lorraine for Pr João F Mano (Portugal) and the satellite laboratory Tissue Engineering and Regenerative Medicine: The bearer of the project, and responsible for the Advanced ERC file
   
• 2015: ERC proof of concept 2017, ERC Advanced 2020, ERC PoC 2020
   
• "Professor@lorraine" is a programme of the ISITE Lorraine Université d’Excellence (LUE) aimed at consolidating world-class collaborations through the multi-year hosting of high-level international researchers
   
• 2014-2021: “ASCATIM: Cardiac assistance” project in the FEDER-FSE Lorraine and Vosges Massif programme relating to the development of a cardiac exoskeleton.“Treatment of Myocardial Infarction" operational programme. Carriers JP Jehl (404) and N. Tran (Surgical School of Nancy Lorraine)
   
• PACTE IMT Grand EST project // TechLab "Biomaterials and Biomimicry" in the funded action entitled "Techlabs and LivingLabs for training in digital technologies in the fields of health and personalized medicine"
   
• Intra-IJL thematic project entitled "Bioinspired biomaterials for tissue engineering and regenerative medicine" (2019-2022) carried out in collaboration with the following IJL groups and competence centers : Micro and Nanomechanics for the Living  ; Physics, Mechanics and Plasticity ; Functional Thin Films for Energy Applications group ; CC MagCryo ; CC X-Gamma, CC 3M
   
• 2015-2017: “2015 Promising researcher”, FEDER- Grand Est Region

Articles :

• Surface Micro‐and Nanoengineering: Applications of Layer‐by‐Layer Technology as a Versatile Tool to Control Cellular Behavior, Small, Volume:  15    Issue:  30      Article Number:  1901228    (2019), MP Sousa, E Arab‐Tehrany, F Cleymand, JF Mano.
   
• Bioactive films containing alginate-pectin composite microbeads with Lactococcuslactissubsplactis:Physicochemical characterization and antilisterial activity, Int. J. Mol. Sci., 19(2), 574 (2018). M. Bekhit, E. Arab-Tehrany, C. J.F. Kahn, F. Cleymand, S. Fleutot., S. Desobry, L. Sánchez-González
   
• Elaboration of hydroxyapatite nanoparticles and chitosan/hydroxyapatite composites: a present status, Polymer Bulletin 1-33 (2018), D Boudemagh, P Venturini, S. Fleutot, F. Cleymand.
   
• Multilayered membranes with tuned well arrays to be used as regenerative patches, ActaBiomaterialia 57, 313-323 (2017), N. I. Martins, M. P. Sousa, C. A. Custódio, V. C. Pinto , P. J. Sousa, G. Minas, F.Cleymand, João F. Mano.
   
• Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels, Int. J. Mol. Sci., 18(12), 2675, (2017), K. Rahali, G. Ben Messaoud, C. J.F. Kahn , L. Sanchez-Gonzalez , M. Kaci, F. Cleymand , S. Fleutot, M. Linder , S. Desobry, E. Arab-Tehrany
   
• Multiscale characterization of the hierarchical structure of Dynasteshercules elytra, Micron 101, 16-24, (2017), C. Roux-Pertus, E. Oliviero, V. Viguier, F. Fernandez, F. Maillot, O. Ferry, S. Fleutot, João F. Mano, F.Cleymand.
   
• Membranes combining chitosan and natural-origin nanoliposomes for tissue engineering, RCS Advances, 6, 83626-83637 (2016), F. Cleymand, H. Zhang, G. Dostert, P. Menu, E. Arab-Tehrany, E. Velot, João F. Mano.
   
• Elastic chitosan/ chondroitin sulphate multilayer membranes, Biomed Mater. 11(3):035008, (2016), MP Sousa, F. Cleymand, João F Mano
   
• Effects of radio frequency (RF) Cold plasma on chitosan and its nanoliposome blend thin films for tissue engineering applications, Carbohydrate polymers, 93(2), 401-411, (2013), Zhang H.Y., Cleymand F., Kahn C.J.F., Linder M.; Henrion G., Dahoun A., Arab-Tehrany E.
   
• Structural, mechanical and barrier properties of active PLA–antioxidant films, Journal of Food Engineering, 110, 2012, 380-389., Jamshidian M., Arab Tehrany E., Imran, M., Akhtar, M.J,Cleymand, F., Desobry, S.
   
• Effects of synthetic phenolic antioxidants on physical, structural, mechanical and barrier properties of poly lactic acid Film, Carbohydrate Polymers. 87 (2), 2011, 1763-1773., Jamshidian M., Arab Tehrany E., Cleymand F., Leconte S., Falher T., Desobry S.

Models for health

The group has developed:

• experimental models such as organoids in the form of hydrogel with human proteins or elaborated by 3D bio-printing. These use novel bio-inks; surfaces with nano/micro-topographic, physically or chemically contrasting patterns produced on a variety of materials. These new platforms could be used to control cell behaviour and drug release profiles or for high-throughput screening of new active ingredients or drugs for applications in regenerative medicine or cancer.
   
• numerical models. The aim is to better understand and model the biomechanical behaviour of the human cornea (Abaqus) to study physiopathologies associated with keratoconus.

Thesis:

Nicolas Falgayrettes (UMI GT Lorraine, CHRU Metz): "Digitization of the Human Cornea" Collaboration with Mercy Hospital (DR JM Perone) / thesis co-direction with GeargiaTech Loraine (Pr E. PatOor),

Projects:

• Cancéropôle Est's "Support for the Emergence of 2019 Projects": New platform to develop humanized 3D in vitro osteosarcoma models for drug screening and validation: Hydrogels based on human platelet lysates (2019 - 2021)
   
• Emerging project 2019 LUE: Study of the Transfer of Active Vectorised Molecules on microfluidic chips, by nanoliposomes through a barrier model (Leader: Libio/Biopôle)
   
• Regional project: Study and optimisation of the biomimetic integration of implant-prosthetic abutments within the marginal periodontal environment (2016-2017)

Articles :

• Fretting-corrosion behavior on dental implant connection in human saliva, Journal of the Mechanical Behavior of Biomedical Materials Volume 94, Pages 86-92 (2019) P.  Corne, P. De March, F Cleymand , J. Geringer
   
• Surface Micro‐and Nanoengineering: Applications of Layer‐by‐Layer Technology as a Versatile Tool to Control Cellular Behavior, Small, Volume:  15 Issue:  30 Article Number: 1901228 (2019), MP Sousa, E Arab‐Tehrany, F Cleymand, JF Mano

Development

• Elaboration of organic and inorganic materials by soft chemistry or solution chemistry.
• Microwave, hydrothermal, co-precipitation, sol-gel, polyol, microemulsion synthesis of nanoparticles, including bare or functionalised magnetic nanoparticles.
• Shaping of biomaterials by solvent casting, spin coating, deep coating, electrospinning, electrospraying and 3D/4D bioimprinting, including the development and optimization of home-made techniques.
• Development of organoids by different techniques, including 3D/4D bioimprinting, layer-by-layer techniques, hydrogels.
• Elaboration and optimization of bio-inks.
• Functionalization of surfaces.

Characterization

Mastery of multi-scale, chemical, physical, mechanical and biological characterizations adapted to biomaterials and nanoparticles: carrying out experiments and interpretations.

• Determination of structures by electron diffraction, X-rays and surface analysis by high-resolution X-ray photoemission (XPS)
• Study of the structural properties and morphologies of surfaces by Atomic Force Microscopy (AFM) and measurement of local mechanical, magnetic and electrical properties by Near Field Spectroscopy (NFS)
• Study of the biocompatibility of materials
• Development of bio-inks and 3D organoids
• Development of biomaterials in all forms (hydrogels, films, fibres, nano/micro capsules, self-supporting membranes, 3D architectures, organic or inorganic nanoparticles)
• Characterization of multifunctional magnetic nanoparticles

Professors, assistant professors

• Franck CLEYMAND
• Solenne FLEUTOT
• Jean-Philippe JEHL
• Emmanuel LAMOUROUX

PhD students

• Lyns Verel CHE DJI
• Bertrand GUIBERT
• Amr RADWAN

Post-doctoral researchers

• Mohamed Amine DJEBBI
• Raoua FATTOUM